AKTIVITAS ANTIBAKTERIAL EKSTRAK DAUN TEMU KUNCI (Kaempferia pandurata) TERHADAP BAKTERI Aeromonas hydrophila
ABSTRACT: This study
aims to assess
the leaf extract
of temu kunci
(Kaempferia pandurata), as an antibacterial of
A. hydrophila. The
experiment was conducted
at the Laboratory
of Aquatic Microbiology, Faculty
of Fisheries and Marine Sciences, University Mulawarman, which began in January
to February 2014. Concentration of leaf
extracts are used in the temu kunci inhibition test order for Agar Disc
Diffusion method (ADD) on the growth of bacteria A. hydrophila is 100 ppm, 200 ppm,
300 ppm, 400
ppm, 500 ppm,
600 ppm, 700
ppm, 800 ppm,
900 ppm, 1000
ppm, 1500 ppm, 2000 ppm, 2500
ppm, 3000 ppm, 3500 ppm, 4000 ppm, 4500 ppm, 5000 ppm, 5500 ppm and 6000 ppm.As
a positive control is antibiotic Amoxicillin and fisiogis solution as a
negative control. Ekstrak dripped on
paper discs, placed
on the media
that has overgrown
BHIA A. hydrophila bacteria. Then further tested with a bacterial culture
test with the extract, the bakteria count using the Total
Plate Count and
Spektrofotomter.Concentration was used
the best ADD
600, 700, 800, 900 ppm, which the best inhibition zone
ranged 10,5-13 mm. The results showed
leaf extracts of K. pandurata are antibacterial against A. hydrophila. In ADD
the best leaf extract concentrations is 900 ppm, while in the bakterial culture
countextract concentration is 600 ppm.
Penulis: SELAMAT HARIYADI, GINA
SAPTIANI dan ESTI HANDAYANI HARDI
Kode Jurnal: jpperikanandd140130
