Spesifitas Antibodi Bovine Zona Pellusida 3 (Anti-bZP3) Terhadap ZP3 Kelinci Berbasis bZP3 Sebagai Antigen Kontraseptif
ABSTRACT: Zonna pellucidae can
be develop as antigen potential candidates based on reversible
immunocontraceptive vaccines. Immunogenic sites of bovine zonna pellucidae 3
(bZP3) could stimulated the presence of anti-bZP3 which be located on rabbit ZP
and inhibit sperm-egg interaction on fertilization process. Purpose of this
research is to detect spesific binding anti-bZP3 to rabbit oocytes using dot
blotting and ELISA method. Sub cutan induction of bZP3 with Freund's adjuvant,
CFA (Complete Freund's Adjuvant) for initial immunization and following by IFA
(Incomplete Freund's Adjuvant) at the 14th day and 39th day. Control female
rabbit injected by Tris-Cl buffer diluted in Freund's adjuvant without bZP3
antigen. Rabbit serum injected to rat for producing Rat Anti Rabbit Anti-bZP3.
This research concludes spesific binding of anti-bZP3 with increasing purple
colour on dot blotting methods. Anti-bZP3 increasing on 24th day and 31th day
and still until 48th day. Measurement with ELISA methods showed increased titer
on OD405. Highest titer showed on 31th day post immunization. Anti-bZP3
synthetized by bZP3 induced on rabbit detectable by immunohistochemistry
methods on late primary oocytes, early secondary oocytes, growing secondary
oocytes, and oocytes on de Graaf folicular phase.
Penulis: Edwin Widodo,
Aulanni’am
Kode Jurnal: jpkimiadd050036
