PRELIMINARY STUDIES FOR PRODUCING CRUDE LIPASE FROM TEMPE’S MOULD CULTIVATED IN RICE-HUSK-BASED SOLID MEDIA
ABSTRACT: The goal of these
preliminary studies is to support Indonesian program for increasing palm oil
added value through independent production technology based on Indonesian
natural resources. Various palm oil derivatives could be synthesized
enzymatically using lipase from microbes that available in Indonesia. Tempe’s
mould is available in abundance in Indonesia and had already been proved for
producing lipase. This paper provides information about producing crude lipase
from Tempe’s mould cultivated in rice-husk-based solid media using palm oil as
carbon source. Observed variables include solid media composition, optimum
fermentation time, extraction and enriching process of crude lipase. The crude
lipase was analyzed its hydrolysis activity on coconut oil and palm oil. The
result of these preliminary studies shows that this production process is a
simple and tough process and very potential to be developed.
Author: Teuku Beuna Bardant,
Kiky Corneliasari Sembiring, and Achmad Hanafi Setiawan
Journal Code: jpkimiagg070023
