Pemanfaatan Protease Termofil yang Hidup di Sumber Air Panas Cangar Batu Malang
ABSTRACT: Thermophile protease
has economic value, because this enzyme is useful on all kind of industry that
used high temperature on it production process. The thermophile has been
isolated from hot water spring at Cangar Batu Malang called CG-10 isolate. The
characteristic of CG-10 are; bolt colony has a brown-white colour, rod form
cels with size 6-14 µm and Gram positive. Identification with 16S-rRNA gene of
their isolate shows 98.305% similarity with B. caldoxylolyticus. The protease
characteristic of CG-10 isolate, fractionated by ammonium sulfate 35% (w/v),
centrifuged by 4000 rpm of speed for 15 minute, (1) has optimum temperature of
80oC; (2) has optimum pH 8, (3) can survive until 60 minutes of incubation time
at temperature 80oC , (4) the molecule weight of: 60-76 kDa with pH1 value
between 7.5 - 8.20, (5) this protease is alkaline serine protease, and (6)Vmaks
value 0.622 unit menit -1 and KM 9.8 µmol/L, so this CG-10 isolate protease can
be use in detergent industry.
Penulis: Rudiana Agustini
Kode Jurnal: jpkimiadd060067
