OUTDOORS BATCH CULTIVATION OF MARINE MICROALGAE Nannochloropsis sp USING PARALLEL GLASS TUBULAR PHOTOBIOREACTOR
ABSTRACT: Microalgae
Nannochloropsis sp is autotrophic organism that can utilize atmospheric CO2 and
sunlight for growth via photosynthesis. It is potential sources for biodiesel
due to its high lipid content. For mass production, two basic designs of
photo-bioreactor are available, i.e. open and enclosed systems. Study was
conducted to investigate the growth performance of Nannochloropsis sp in outdoor batch culture
system using Parallel Enclosed Glass-Tubular Photo-bioreactor (PBR) for 18 days
with modified f/2 medium. Nutrient N-P was calculated based on the empiric
structure of microalgae cell (C1O0.48H1.83N0.11P0.01) with target of biomass
yield was 1g.L-1. Temperature, pH, Optical Density (OD), Dry Cell Weight (DCW),
population, lipid content, and fatty
acid were observed. Data showed that temperature fluctuated between 22oC and
35oC, pH is stable at 7.0-7.5, OD increased from 0.134 to 0.878, DCW increased
39 times higher than its initial value.
At 18th day, the cell diameter varied in range of 1-9 µm, cell weight
increased 5.3 times (3.5 to 18.4pg.cell-1), and lipid content is 35.71%. Fatty
acid was composed by saturated fatty acids (92.39%) that covered of Lauric
(20.30%), Myristic (12.69%), Palmitic (50.05%), and Stearic (9.35%) that
suitable for biodiesel. Unsaturated fatty acid was performed by Oleic acid
(3.49%).
Author: Astuti, J.T.,
Sriwuryandari, L., Priantoro, E.A., dan Sembiring, T.
Journal Code: jpbiologigg120006
