Isolasi dan Identifikasi Senyawa Flavonoid Fraksi Etil Asetat Rimpang Tumbuhan Temu Kunci (Boesenbergia pandurata (Roxb) Schelecht) (Zingiberaceae)
ABSTRACT: Boesenbergia
pandurata (Roxb.) Schlecht is one of fingerroot plant in ginger family
(Zingiberaceae). The rhizomes of the plant contained a lot of secondary
metabolites compounds. Therefore, the purpose of the research is to isolate and
identify the flavonoid compound from the plant. The rhizomes were extracted
with metanol continued by partition using ethyl acetate-water (1:1). The ethyl
acetate extract was chromatographed on a column of Si gel (Vacuum Liquid
Chromatography and Gravitation Column Chromatography) using n-hexane-ethyl
acetate (5:2) as eluents. Further purification by recristalization using benzene
produced a compound as yellow powder (16 mg) having melting point of
294-295 oC. The spectra of isolated compound were determined by spectroscopic
UV-Vis, FT-IR, and GC-MS. Spectrum UV-Vis of the isolated compound showed
ultraviolet absorption at λmax (MeOH, nm) 290 and 322; λmax (MeOH+NaOH, nm)
322; λmax (MeOH+AlCl3, nm) 309; λmax (MeOH+AlCl3+HCl, nm) 310; λmax
(MeOH+NaOAc, nm) 322 and λmax (MeOH+NaOAc+H3BO3, nm) 290. Its FT-IR spectrum
represented a number of absorption lied on νmax (cm-1) : 3142.5; 3012.6; 2893;
2345.3; 1631.7; 1585.4; 1357.8; 1168.8; and 825.5. GC-MS spectrum of the
isolated compound exhibited an [M]+ ion peak at m/z = 256 with retention time
of 22,579. Based on the results of
spectrum analysis it can be concluded that the compound is
5,7-dyhydroxyflavanone.
Penulis: Ochtavia Prima Sari,
Titik Taufiqurrohmah
Kode Jurnal: jpkimiadd060069
