Imobilisasi Enzim Glukosa Oksidase dari Penicillium sp-3 Galur Lokal
ABSTRACT: Immobilization of
glucose oxidase (GOD) enzyme from Penicillium sp-3 local strain has been
carried out using ionotropically entrapping method in Ca-alginate membrane coupled with
Na-polyacrilate. The entrapment of the enzyme in diffusion membrane occur
spontaneously by cross-linking between Na-alginate/Na-polyacrilate and CaCl2.
The GOD enzyme immobilized by addition of 1 % Na-polyacrilate has the highest
encapsulation efficiency, that is 87.13 % with the smallest percentage of
diffusion, i.e. 23.37% and the relative activity of 50%. The GOD immobilized
enzyme had good stability at the pH range 4 - 7 during 30 minutes of storage
and was stable at a temperature of 20 oC. The activity of the GOD enzyme after
being utilized continuously for 5 times only decrease up to 47,06 % compared to
that in the initial utilization.
Penulis: Ahyar Ahmad, Andi
Syaiful, Firman AP Firman AP, Abdul Rauf Patong
Kode Jurnal: jpkimiadd070089
