Aktivitas Antioksidan dan Antibakteri Pigmen Bixin Selaput Biji Kesumba (Bixa orellana L.)
ABSTRACT: Research on Bixa
orellana L. have been done to isolate, identify and determine bixin percentage,
the antioxidant and antibacterial activities of bixin from B. orellana
seed. Isolation and identification of
bixin was done by thin layer chromatography (TLC), column chromatography,
chemical test of bixin and UV-Vis double beam spectroscopy. Percentage of bixin
was calculated by JECFA method, the antioxidant activity was determined by DPPH
(1-1 diphynilpicrylhidrazil) method while antibacterial activity was analyzed
by the use of agar diffusion method. Thin layer chromatography (TLC) for the
crude extract contained 5 spot, where spot 5th was bixin. Bixa orellana has
75±3% of bixin. Antioxidant activity of bixin had IC50 548.5±20.0 ppm. Whereas
the antibacterial activity of bixin against the Escherichia coli and
Staphylococus aureus could be classified as weak inhibition category at 500-750
μg and medium inhibition category at 1500 μg.
Penulis: Pipin T. Kurniawati,
H. Soetjipto, Leenawati Limantara
Kode Jurnal: jpkimiadd070088
