GENERATION OF ARABIDOPSIS THALIANA LINES WITH SUPPRESSED ATNDA1 UTILIZING RNA INTERFERENCE
Abstract: Atnda1 is
one of matrix
NAD(P)H dehydrogenase genes
in Arabidopsis thaliana.
Its function is
still unclear. This is an
encouragement to study the gene by disrupting the expression of the gene. This
study was aimed to generate Arabidopsis
thaliana possessing suppressed Atnda1 utilizing RNA interference. Double stranded nda1-RNAi silencing was
chosen for this project. The generation of the lines was started by creating
specific primer for Atnda1. The
creation of nda1-RNAi
cassette used the
Gateway cloning. We
utilized Agrobacterium strain GV3101
as a mediator
to transfer the
cassette into the
plants. Dipping the
flower into Agrobactrium
solution was done to
transfer the nda1-RNAi
cassette into wild
type Arabidopsis. The
seeds collected from
the mutant plants were checked in
selective media. Contamination was the daunting problem in the process of
checking the plants. This was
crucial to ensure
that the insertion
has been done
successfully. However, selection
of the mutants must be done
before analysis of the function is carried on the mutants.
Author: Wardiah
Journal Code: jpbiologigg120004
