Biocompability of Low Dose of Human Menopause Gonadotropin (hMG) and Deglycosilation of Human Menopause Gonadotropine (dGhMG) Against Bovine Eggs
ABSTRACT: No any researcher
yet reported by adding gonadotropin mainly either hMG or dGhMG less than 1
ug/ml into media succesfully obtain proportional amount fertilized eggs in
invitro fertilization technique (IVF), moreover this dosis twice and four times
decreased from 1000 ng. The main purpose of this study was to evaluate
biocompatibility of low dosis of hMG and dGhMG as additive in media maturation
during in-vitro maturation (IVM) to amount of fertilized egg obtained following
48 hrs of in-vitro insemination (IVI). All those inclusive criteria oocytes
that collected in laboratorium invitro Lab. obstetrics Department of
Reproduction, Vet medicine,Unair randomized into sub-group (1, 2, 3, and 4) and
also control group. Sub-group 1, 2, 3, 4 and control group Containing with
TC199 + 10% bovine calf serum. Sub-group 1 added with hMG 250 ng/ml and 500
ng/ml for sub-group 2. Sub-group 3 and 4 added with 250 ng/ml and 500ng/ml
dGhMG respectively . all these immature oocytes were transfer into those each
drop 50ul media in 35 mm disposible petridisk and contained each drop 5–7 oocytes then covered in with mineral oil and polyesterene
plastic petridisk cover then incubated for 24 hrs in 5% CO2 incubator. Next
morning after oocytes washed in warm Media oocyte washing solution (OWS) and
once using Earle’s balanced salt solution (EBSS)
all predicted mature oocytes transfered into centripetal side of Rosset patern
made using EBSS media 10ul each , that connected each other to centre of
centripetal side 50ul .This centre side of centripetal was transfered with 30
ul semen have been followed and washed twice then swim-up process then kept in
5% CO2, 38.5° C for 24 hrs. Observation performed 2 × 24 hrs after all oocytes
transfer into maturation media 24 hrs following IVI . All sub-group showed
increasing amount of fertilized eggs higher than 75% and much higher compared
to control group that only 38.1%, but among subgroup was not much differed, however
the other hand found highest fertilized eggs/embryo was 87.5% in sub-group 4.
Wishing to reach more fertilized eggs in IVF, gonatropine hormone absolutely
should be added in basically maturation media.
Author: Mahaputra L, S
Koesdarto, HA Hermadi, Srimulyati
Journal Code: jpbiologigg120001
