VARIASI FENOTIP DAN GENOTIP EBONI (Diospyros celebica Bakh) PADA HUTAN ALAM DAN HUTAN TANAMAN DI SULAWESI TENGAH DAN SULAWESI BARAT
Abstract: The aim of this
research are to know phenotype and genotype diversity between population of
ebony on the forest in Sulawesi specially from Central Sulawesi and West
Sulawesi. The study was conducted from January to April 2014, extraction
of DNA has been done in laboratory
of Biotechnology, faculty of Mathematics
and Natural Science, Tadulako University and DNA analysis in Kyoto Perfectural
University, Japan. PCR-RAPD technique was employed in this study with 4 RAPD
primers. Sample from 9 population in natural forest and plant forest in Central
Sulawesi and West Sulawesi and all leaf sample taken show different. There were
2 primary that resulting the best amplifying quality in genotype diversity
analysis that was TCH05 and AS9870. There is unique bands on DNA fragment from
Lende area, has band size 2500-3000bp while from ebony individu sample from
other area locus cannot be found. Based on dendogram analysis on distance
matrix revealed the 10 genotypes were grouped into two main groups. The first
group population from Lende. The second group was further divided into two sub
group (2A and 2B). Subgroup 2A consisted of Diospyros kaki. Subgroup 2B
included population from Ako, Tibo, Bale, Tompe, Maleali, and Kasimbar.
Penulis: Wahyuningsih, Muslimin,
Yusran
Kode Jurnal: jpkehutanandd140073
