Uji Aktivitas Penghambatan Enzim Alfaglukosidase Pada Beberapa Tanaman Suku Euphorbiaceae
Abstract: Diabetes mellitus
(types 1 and 2) is recognized as a serious global health problem that
characterized by hyperglycemia. Type 2 diabetes is more common in diabetic
populations. In type 2 diabetes mellitus, inhibition of α-glucosidase is a
useful treatment to delay the absorption of glucose after meals. Avoiding the
adverse effects of current agents, it is still necessary to search alternative
for better options. Plants have been a rich source of α-glucosidase inhibitors.
In this research, screenings based on chemotaxonomic approach to determine the
class of chemical constituents and to know
α-glucosidase inhibiting activity of some plants from Euphorbiaceae. The
simplisia powder was extracted using ethanol 80% by reflux. Measurement of
inhibitory activity of α-glucosidase
performed using a spectrophotometer UV-VIS. In vitro assays of α-glucosidase activity showed 14 extracts had
IC50 values of between 2.34 µg/mL and 64.78 µg/mL, which were lower than
that of acarbose (117.20 µg/mL). Leaves extract from Antidesma celebicum had
the highest α-glucosidase inhibiting activity with an IC50 of 2.34 µg/mL. The results of
phytochemical screening in 15 extracts generally contain glycosides,
terpenoids/ sterols, tannins, saponins and alkaloids.
Penulis: Berna Elya
Kode Jurnal: jpfarmasidd120148
