UJI AKTIVITAS PENANGKAPAN RADIKAL DPPH EKSTRAK PRODUK TEH HITAM (Camellia sinensis (L.) O.K.) DAN GAMBIR (Uncaria gambir (Hunter) Roxb) SERTA PROFIL KLT-DENSITOMETERNYA
Abstract: The purpose of this
research was to compare the DPPH scavenging activity of black tea (Camellia
sinensis (L.) OK) and gambier (Uncaria gambir (Hunter) Roxb) product extract
and to determine their TLC-densitometer profile.
This research was conducted in two stages, first stage was to examine the
DPPH scavenging activity of black tea and gambier product extract. This assay
used some concentrations of black tea product extract, gambier product extract,
and standard vitamin C (10, 20, 40, 80, 160, 200, and 320 µg/mL). The second
stage was to determine the extracts profile by TLC-densitometer.
The result of this research showed that the DPPH scavenging activity of
gambier product extract was greater (IC50 = 88,57±0,64 µg/mL) than black tea
product extract (IC50 = 311,54±2,79 µg/mL). The result of profile determination
of black tea product extract at a mobile phase of MeOH:toluene (50:50) showed
that no spot was supposed a volatile oil group; at mobile phase of ethyl
acetate:formic acid:acetic acid:H2O (100:11:11:26) showed two spots that was
supposed a flavonoid group, spot with Rf 0,41 (?max 365 nm) and spot with Rf 0,97
(?max 280 nm); at mobile phase of CHCl3:MeOH (90:10) showed that spot with Rf
0,87 (?max 275 nm) was supposed an alkaloid group; at mobile phase of acetic
acid:diethyl ether:n-hexane:ethyl acetate (20:20:20:40) showed that spot with
Rf 0,62 (?max 290 nm) was supposed a tannin group. While the result of profile
determination of gambier product extract at mobile phase of ethyl
acetate:formic acid:acetic acid:H2O (100:11:11:26) showed two spots that was
supposed a flavonoid group, spot with Rf 0,81 (?max 280 nm) and spot with Rf
0,92 (?max 280 nm); at mobile phase of acetic acid:diethyl ether:n-hexane:ethyl
acetate (20:20:20:40) showed that spot with Rf 0,42 (?max 285 nm) was supposed
a tannin group.
Penulis: Jaya, I. G. N. I. P,
Leliqia N. P. E, I.N.K. Widjaja
Kode Jurnal: jpfarmasidd120159
