STUDI PENDAHULUAN DERIVATISASI BEBERAPA SENYAWA DENGAN GUGUS AMIN MENGGUNAKAN DANSIL KLORIDA PADA KLT-SPEKTROFOTODENSITOMETER
Abstract: Advance
derivatization study of several amines with dansyl chloride has been carried
out. The aim of this study was to determine influence of molecul structures
compound toward dansylation. Derivatization was done by reacting 4000 ng drugs which contain primary or
secondary amine group with 100 ng dansyl chloride at a temperature 450 C and pH
9 for a hour. Interference was separated using stationary phase silica G60 and
mobile phase etyl acetat : methanol : ammonia
(85:10:5), detection on spectrophotodensytometer ?eks 312 nm mode fluorescence
with filter K400.
Compounds that have a primary or secondary amine groups can be
derivatized using dansyl chloride though
that most affects of the reaction is rigidity of molecul structure. Differences
fluorescence intensity between each derivatives influenced by the reactivity
amine groups and steric hindrance of the compound.
Keywords: amine group, dansyl
chlorida, derivatization, molecul structures, TLC-spectrophotodensytometer
Penulis: Dewi Puspita Apsari,
Ni Putu Linda Laksmiani, I.N.K. Widjaja
Kode Jurnal: jpfarmasidd120158
