UJI AKTIVITAS ANTIOKSIDAN MINYAK BUAH MERAH (Pandanus conoideus LAM.) SECARA in Vitro DAN In Vivo PADA TIKUS YANG DIBERI BEBAN AKTIVITAS FISIK MAKSIMAL
Abstract: Increasing oxygen
consumption during intensive physical exercise may increase production of free
radicals, and if it exceeds physiological capacity may cause oxidative stress
as shown as chance of MDA level. Buah merah oil contains high betacarotene and
tocopherol, as well as fatty acid such
as oleic acid,
linoleic acid, linolenic
acid and decanoic
acid. Tocopherol is major
biological lipid-soluble antioxidant,
protecting structures and
function of cell membranes from
free radicals. The
objective of this
study was to
know in vitro
antioxidant activity and the
effect of buah
merah oil on
MDA level in
blood during maximum
physical activity treatment. In vitro antioxidant activity test was
conducted using DPPH method (2,2-diphenyl-1-picryl hydrazyl).
In vivo antioxidant
activity test was
done by using
24 male Wistar rats in pre - post
test control group design. The rats were grouped into 4 groups. The control
group was given destillated water and three treatment groups were given buah
merah oil in the dose of 0,15 ml/kgBW; 0,3 ml/kgBW; 0,6 ml/kgBW respectivelyfor
10 days. Before buah merah oil was given, level of MDA was measured. Ten days
later, the four group were given maximum physical activity mean of swimming
until the sign of fatigue occurred (nearly drowned) and
the blood was
taken for blood
MDA examination. In
vitro, the IC50
of buah merah oil was 451,51
μg/ml. In vivo test, dosage 0,15 ml/kgBW could decrease MDA 5,22% Dosage 0,3
ml/kgBW could decrease MDA 11,96% and dosage 0,6 ml/kgBW could decrease MDA 8,19%
. Buah merah
oil showed antioxidant
activity in vitro.
In vivo experiment,
all dosage of buah
merah oil decreased
blood MDA level
but in the
dose of 0,3
ml/kgBW decreased MDA level more than dose of 0,15 ml/kgBW and 0,6
ml/kgBW.
Penulis: Ni Made Dwi
Sandhiutami, Ngatidjan, Erna Kristin
Kode Jurnal: jpfarmasidd100034
