PENGARUH VARIASI KEPOLARAN FASE GERAK ASETON-DIKLOROMETANA: METANOL-ASAM ASETAT TERHADAP % DISTRIBUSI (+)-KATEKIN DARI GAMBIR DENGAN METODE KROMATOGRAFI CAIR VAKUM
Abstract: The research was
conducted to determine the influence of mobile phase polarity of
acetone-dichloromethana : methanol-acetic acid on the percent distribution of
(+)-catechin from gambier by vacuum liquid chromatography and to determine the
polarity mobile phase required to obtain the largest amount of (+)-catechin
percent distribution.
Gambier extract was fractionated using vacuum liquid chromatography with
several variations of polarity of acetone-dichloromethane : methanol-acetic
acid mobile phase expressed by the dielectric constant value (?) of 13.74 to
17.51. Fractions were analyzed by TLC-spectrophotodensitometer to calculate the
concentration and the percent distribution of (+)-catechin. To know the effect
of variations in mobile phase polarity on the percent distribution of
(+)-catechin performed statistical analysis using one-way ANOVA test (? =
0.05).
The results show that the variation of polarity of
acetone-dichloromethane : methanol-acetic acid mobile phase (? = 13.74 to
17.51) had a significant influence on percent distribution of (+)-catechin from
gambier by vacuum liquid chromatography method (p <0.05). Fraction with a
mobile phase of acetone-dichloromethane (4:6) 50% : methanol-acetic acid (3:4)
50%, which had a value of dielectric constant of 15.62 yield the largest amount
of (+)-catechin percent distribution value (45.40%).
Penulis: Era S.Y.,, Eka L,
I.N.K. Widjaja
Kode Jurnal: jpfarmasidd120154
