Efek Antimikroba dari Kapang Endofit Ranting Tanaman Biduri
Abstract: Didymella bryniae
strain MA71 endophytic fungus was isolatedfrom twigs of Biduri plant
(Calotropis gigantea L.Dryand). It has anti yeastactivity and anti bacterial
activity against gram positive and negative bacteria.Secondary metabolites were
isolated via fermentation process using PDYmedia for 12 hr. Anti microbial
activity of these fermentation product was assessedvia agar diffusion method
using disk paper. Test result demonstratedthat extracellular secondary
metabolites which was extracted using EtOAcwere active towards Staphylococcus
aureus ATCC 25923, Escherichia coliATCC 25922 and Bacillus subtilis ATCC 6633.
On the other hand, these EtOACextracted metabolites were also active against
Candida albicans ATCC 10231.Its inhibitory zone were 17.3 mm, 17.2 mm, 17.41 mm
and 11.6 mm respectively.Chloramphenicol was used as positive control for
bacteria and nystatinfor yeast. Overall, the se condary metabolites produced by
fermentation of endophyticfungi Didymella bryniae strain MA71 have broad
spectrum of antibacterial and yeast activity.
Keywords: endophytic Fungus,
shaked fermentation, secondary metabolites,antimicrobials, and Calotropis
gigantea L. Dryand
Penulis: Shirly Kumala
Kode Jurnal: jpfarmasidd140119
