UJI BAKTERI ESCHERICHIA COLI YANG RESISTAN TERHADAP ANTIBIOTIK PADA IKAN KAPAS-KAPAS DI SUNGAI BATANG ARAU PADANG

Abstract: A study in antibiotic resistance Escherichia coli from kapas-kapas fish in Batang Arau River has been achieved. This work is to determine the presence of pathogenic bacteria (such as E.coli) by Most Probable Number (MPN) method and bacteria resistant against antibiotics as well. From the qualitative test there were positive coliform and fecal coliform bacteria. It was obtained that there are 333 combination which ≥ 2400/100 mL, and 332 about 1100/100 mL, respectively based on MPN method according to Mc Crady. The result of isolation showed that there are five cultures E.coli found by the biochemical test. It was shown that methyl red and lactose posses positive E. coli, while citrate was negative. The antibiotics halozone showed Meropenem which indicates highest sensitivity to E.coli. All of antibiotics pattern were resistance and intermediate (30%) and susceptible (40%). The amplification result indicates Escherichia coli O157: H7 was not found by using stx1 gene.
Key words: Escherichia coli, kapas-kapas fish, Antibotic resistance, Electrophoresis
Penulis: Rina Anggraini, Marniati Salim, dan Elida Mardiah
Kode Jurnal: jpkimiadd130218

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