ISOLASI DAN KARAKTERISASI SENYAWA TRITERPENOID DARI FRAKSI AKTIF SITOTOKSIK BRINE SHRIMP LETHALITY BIOASSAY DARI EKSTRAK DAUN MIMBA (Azadirachta indica)
Abstract: Isolation and
characterization of triterpenoid from leaves of neem (Azadirachta indica) have
been carried out. Sample extracted with maseration method using n-hexana, ethyl acetate and methanol. The
Brine Shrimp Test proved that n-hexana fraction as active fraction of
cytotoxic. Active fraction purified by column chromatography method using
silica gel as the stationary phase and n-hexana, ethyl acetate as the mobile
phase. Isolated compound suggest as triterpenoid compound which have melting
point is 151,8 – 152,3ºC and show single spot with some kind of eluent.
Ultraviolet’s spectrum refer to presence of double bond (not conjugate) and
Infrared’s spectrum show that compound have C=O carbonyl, C-H stretching, C-O
alchocsi, O-H and specific absorban of triterpenoid is geminal dimetil.
Penulis: Viona Yolanda, Sanusi
Ibrahim, dan Mai Efdi
Kode Jurnal: jpkimiadd130219
