Penentuan Kadar Etanol Pada Sampel Minuman dengan Metoda HPLC Menggunakan Fasa Gerak Asetonitril dan Buffer Fosfat
Abstract: Alcoholic beverage
is a drink containing ethanol. Ethanol is a psychoactive substance and its
consumption causes loss of consciousness. All types of alcohol are essentially
toxic, especially if consumed in excess, such as ethanol. According to BPOM limit of the use of alcohol in drinks
are ± 1% - 5%. Ethanol content analysis in this study using HPLC method with
condition UV-Vis using a mobile phase of acetonitrile: phosphate buffer (5: 95)
pH 6 at 220nm wavelength detector and
the use of stationary phase Zorbax Rx C18 ODS column. The result of this
method is retention time for ethanol
1.68 minutes. The aplication of the method for determination of ethanol on the
drinks from the market for 5 different samples was tested. The sample consists
of 3 types of alcoholic beverage production factory/industrial, and 2 types of
alcoholic beverages produced household. With adition standard techniques on
samples was obtained ethanol adduct levels in 2 samples are 20.416% and 3.80%.
Penulis: Nailul Rahmi, Budhi
Oktavia, Nazulis Z
Kode Jurnal: jpkimiadd130471
