Isolasi dan Karakteristik Bakteri Pendegradasi Selulosa pada Ampas Tebu Kuning (Bagasse)
Abstract: Cellulose is the
main carbohydrate synthesized by plants and it occupies almost 60% of the
components of the structure of plants. Natural
process of decomposition of cellulose requires the help of
microorganisms that secrete the
enzyme cellulase. Bagasse
contains 32-34% cellulose.
This study aims
to determine the cellulose
degrading bacteria of yellow
bagasse and know the ability of the
bacteria to degrade cellulose bagasse. The
samples were taken
from ice-cane seller
in the District
of South Pontianak.
To isolate of cellulose degrading bacteria, the dilution
method of Carboxymethylcellulose (CMC)
was used and HC test was performed on
solid CMC. Dry
weight of test
was performed using
a fresh bagasse
as a source
of cellulose. The results showed that there are fifteen pure isolates of
cellulose degrading bacteria consisting of
eight genera, namely Psedomomas,
Bacillus, Nocardia, Kurthia, Vibrio, Flavobacterium, Neissera, and Micrococcus. AT3BPS2 isolates (Nocardia)
has the largest ratio of 2.67 HC. Isolates that have the lowest HC constellation
is AT1BPS1 isolates
(Pseudomonas) 1.06. Isolates
that has the
highest cellulose degradation
ability is AT1BPS2 (Pseudomonas) at
19.28% and isolates with the lowest degradation ability is AT3BPS2 (Nocardia)
at 7.21%.
Penulis: Krispina Nofu, Siti
Khotimah, Irwan Lovadi
Kode Jurnal: jpbiologidd140101
