IDENTIFIKASI VARIASI GENETIK PARIJOTO (MEDINILLA JAVANENSIS(BL.)BL.DAN MEDINILLA VERRUCOSA (BL.) BL.) DENGAN PENANDA MOLEKULAR SEBAGAI SUMBER BELAJAR
Abstract: Indonesia as a
megabiodiversity country have to explore biodiversity from the ecosystem level
to genetic variations to avoid loss of them. Parijoto is ones of Indonesian
plant used for ornamental plants and medicine. Parijoto included in genus of
Medinilla. Ten species has been identified scattered on the mountains of Java.
There are two cultivated species (Medinilla javanensis and M. verrucosa) in
Muria mountainous, Central Java.Both of them have similar morphological
character, therefore they often make confuses. On the other side, the genetic
information of them is needed. Hence, on the level of gene is necessary to
identify M. javanensis and M. verrucosa by molecular markers. The purposes of
identification using molecular markers are to distinguish both of two spesies
and get their genetic information. Identification on a level genes carried by
variation sequence of DNA (polymorphism) between M. javanensis and M.
verrucosa. Method of Restriction Fragment Length Polymorphism (RFLP) and Random
Amplified Polymorphism DNA (RAPD) are usefull to identify genetic variation
between organisms. The use of them should show polymorphism after observed
using electrophoresis method. The appearance of polymorphism shows variations
on the genetic level to strength the identification through morphological
character. Result of the research will be applied as a learning material of
high school students to explain genetic diversity. Students expected to
understand genetic variations not only as an image on book, but in contextual
way.
Penulis: Yasir Sidiq
Kode Jurnal: jpbiologidd140226
