PENGARUH PEMBERIAN CHITOSAN TERHADAP KADAR GLUKOSA DARAH DAN HISTOLOGI PANKREAS TIKUS Sprague dawley YANG DIINDUKSI ALOKSAN
Abstract: Diabetes Mellitus
(DM) is a disease that will increase in the future, and most of them occur in developing
countries. In addition, DM also causes many complications. Therefore, the
alternative medi-cation is needed to help treatment. One of the substances
suspected having hypoglycemic effects is chitosan. Researcher find that
chitosan oligosaccarides (hydrolysis of chitosan) have a dietary fiber and
hypoglycemic effect on the Wistar rat. However, the hypoglycemic effect of
chitosan itself is not yet known. This research was conducted to determine the
hypoglycemic effect of chitosan. The aim of this research was to explore the
effect of chitosan on blood glucose level and histology of pancreas in diabetic
Sprague dawley rat induced by alloxan. Fourteen Sprague dawley male rats, aging
2 months, weighing 141-201 grams were divided randomly into 2 treatment groups.
Group I (P1) were given alloxan 80 mg / kg BW i.v until diabetic and then
chitosan 500mg/kg BW; group II (P2) were given alloxan and then metformin
9mg/dose. Both groups were provided with standard diet and drinking water ad
libitum. In day 0, 7, 14, 21, and 28 blood glucose level was measured. The rats
were sacrified after 28 days treatment then the pancreatic organ taken for the
histologic examination. The slide had been made using paraffin method and
smeared by Kromium Hematoxilin Floxin. Slides were analyzed by measur-ing the
islet’s diameter and the number of pancreatic รข cell. Result of this research
showed that chitosan decreased blood glucose level every week in DM rats (p
<0.05). There was not any significant difference of blood glucose level
between the two groups on the measurement on day 0 and 7 (p> 0.05) and there
was significant differences of blood glucose level between the two groups on
the measure-ment on day 14, 21, and 28 (p<0,05). Diameter size and the
number of รข cells in both groups did not have significant differences (p>
0.05). This research conclude that oral administration of chitosan 500mg/kg BW
had similar hypoglycemic effect, even better compared with the metformin
9mg/dose.
Penulis: Farida Nur Isnaenia,
Lily Arsantib, Woro Rukmi Pratiwic
Kode Jurnal: jpkesmasdd110025
