Uji Aktivitas Antimikroba Ekstrak Etanol Daun Cocor B ebek (K alancho e pinnata) ter hadap Bakteri Staphylococcus aureus Atcc 6538 dan Escherichia coli Atcc 11229 Secara Invitro
Abstract: Cocor Bebek leaves
(Kalanchoe pinnata) contains cinamic acid, flavonoid, alphatocopherol dan
bufadienolide acid which are presumably able to impede a bacterial growth so
that the ethanol extract of cocor bebek leaves are indicated having an
antimicrobe effect. This research purposes to find out the existence and
nonexistence of the impeding power of the ethanol extract of cocor bebek leaves
(Kalanchoe pinnata) on the
Staphylococcus aureus and Escherichia coli bacterias growth. The
research is laboratory experimental with the ethanol extract of cocor bebek leaves
(Kalanchoe pinnata) as the research subject. Bacterias which are used are
Staphylococcus aureus ATCC 6538 and
Escherichia coli ATCC 11229. The
research method is Kirby Bauer by using an oxoid disk. The first step is standardizing each of 24
hours-aged Staphylococcus aureus dan Escherichia coli on BAP and Mc. Conkey
medias in the standard of 0.5 Mc.Farland, then smearing using a sterile
cotton-rid on Muller Hinton media. The researcher uses an empty oxoid disk as a
negative control, an amoxicillin
antibiotic disk on Staphylococcus
aureus and a chloramphenicol on
Escherichia coli as a positive control, while the researcher also places the
oxoid disk containing the ethanol extract of cocor bebek leaves (Kalanchoe
pinnata) with 20%, 40%, 60%, 80% and 100% concentrations on the top of the
plates. Then the researcher measures the impeding zone which is formed after
the incubation on 370°C for 1x24 hours. After that, the researcher analyzes the
data using Mann-Whitney Non Parametry Test. The result is that on the degrees
of 80% and 100%, Staphylococcus aureus bacteria has a significant difference
(p<0,05) from the positive and negative controls. In conclusion, this
research proves the existence of the antibacteria effect of the ethanol extract
of cocor bebek leaves (Kalanchoe pinnata)
on the Staphylococcus aureus
growth in the concentrations of 80% and 100% and the nonexistence of the
antibacteria effect on the Escherichia coli growth.
Keywords: ethanol extract, cocor bebek leaves
(kalanchoe pinnata), antibacteria,
staphylococcus aureus, escherichia coli
Penulis: Ratih Pramuningtyas,
Rahadiyan W.B.
Kode Jurnal: jpkedokterandd090050
