Manfaat Sirih Merah (Piper crocatum)sebagai Agen Anti Bakterial terhadap Bakteri Gram Positif dan Bakteri Gram Negatif
Abstract: Background of
choosing the subject: In recent years world society including Indonesia tend to
consider the use of herbal medicine - back to nature. The advantages of herbal
medicine become an interesting discussion. Most information expand in society
is empirical rather than scientific evidence. Thus red betel vine's benefit
also becomes interesting discussion but evidence based medicine about it is
very little. The purpose of this study is to know the potency of antibacterial
power of ethanol extract of red betel vine (Piper crocatum) toward gram
positive and gram negative bacteria.
Research Methodology: This research was laboratory experimental study.
The extract of red betel vine was made with standard ethanol solvent. This
extract was examined in Staphylococcus aureus ATCC 25923 and Escherichia coli
ATCC 35218. Experimental method is serial dilution. Result was analyzed
descriptively with Minimum Inhibitory Concentration (MIC) and Minimum
Bactericidal Concentration (MBC). The examination in each bacterium was
repeated 5 times.
Result: The result shown that ethanol extract of red betel vine (Piper
crocatum) has ability to inhibit the growth of Gram positive (Staphylococcus
aureus) and to kill it on concentration
25%. While the ability to inhibit the growth of Escherichia coli (Gram negative)
on 6,25% (visual observation and after has been planted in Mc Conkey media).
From result repetition in 5 times, gram negative is more stable rather than
gram positive.
Conclusion: The ethanol extract of red betel vine (Piper crocatum) has
antibacterial effect toward Gram positive and gram negative. MIC and MBC
Staphylococcus aureus at 25% while for
MIC and MBC Escherichia coli at 6,25%.
Keywords: antibacterial – Red
betel vine – Piper crocatum –
Staphylococcus aureus –
Escherichia coli
Kode Jurnal: jpkedokterandd090016
