Gambaran Anatomi Mikroskopik dan Kadar Malondialdehida pada Hati Mencit setelah pemberian Minyak Kelapa Sawit Bekas Menggoreng
Abstract: Palm oil has been
widely used in Indonesia as cooking oil. Repeated heating at high temperatures will damage the cooking
oils by the formation of peroxide, aldehyde, ketone, alcohol, and hydrocarbon
compound. Previous studies reported that giving cooked and oxidized cooking
oils to livestocks has resulted in various poisonous phenomena. To examine
liver damage caused by using cooked palm oil, 36 male Balb/c mice are divided
randomly into 6 groups (n = 6), and to each group were administered orally: (1)
water, (2) fresh palm oil, (3) 4 times cooked palm oil, (4) 10 times cooked
palm oil, (5) 20 times cooked palm oil, and (6) 40 times cooked palm oil; using
gavage needle at a dose of 0,5 ml/100 g body weight/day, once a day for 7
successive days. On the eighth day, part of the liver of each mouse was
examined histologically, stained using Hematoxylin-Eosin to count the number of
necrotic hepatocytes in 20 centrilobular regions randomly. The number of
neutrophils stained using Naphthol AS-D chloroacetate esterase were also
counted in 20 centrilobular regions randomly. The other part of the liver was
homogenized to determine the concentration of malondialdehyde (MDA) using TBARS
method. The results show a significant increased (p<0,05) in the number of
necrotic hepatocytes, the number of neutrophils, and the concentration of MDA
of the groups treated with 10, 20, and 40 times cooked palm oil compared with the group treated
with fresh palm oil. Further studies
about the toxicology of cooked palm oils are warranted to elucidate the
biomolecular basis underlying the xenobiotic metabolism and the defence
mechanisms of the liver.
Penulis: Oeij ., Anindita
Adhika, Wahyuni Lukita Atmadja, Sadiah Achmad, Aming Tohardi
Kode Jurnal: jpkedokterandd0700149
