Efek Antiinflamasi dan Keamanan Phyllanthus niruri L. Herba dan Taraxacum officinale Weber et Wiggers Herba terhadap Dermatitis Alergika pada Mencit
Abstract: Allergic dermatitis
is a common disease in community, and is
still a health problem because of its recurency. Phyllanthus niruri L. Herba
and Taraxacum officinale Weber et Wiggers Herba are often used in treating Atopic Dermatitis (AD). The objectives
of this research are to determine antiinflammation effect of n-hexane fraction
of these herb to Allergic dermatitis in mice and its safety. A longitudinal prospective research
using Randomized Complete Design with Swiss Webster adult male mice strain as
experimental animal. The examination of the antiinflammation effect use 30 mice
divided in 6 group (n=5). Each gruop induced by Ovalbumin at day 1,7, and 21,
except the negative controle group. N-hexane fraction of these herb was given
orally at day 21. The measured data consitsts of the inflammation coverage area, the number of
inflammation cells microscopically at histology preparate with HE and the
percentage of eosinophyl in peripheral blood swab. The acute toxicity test was carried on 80 male mice and 80 female mice . There are differences in
the inflammation coverage area and the
number of inflammation cells on the mice induced by Ovalbumin group (positive
control, 27,30 mm2, 247, p<0,05) and the mice group received Ovalbumin and
n-hexane fraction of the two herbs with dose 1 (12,85 mm2, 142), dose 2 (5,09
mm2, 53), and dose 3 (15,28 mm2, 116). Eosinophyl percentage on the test group
with dose 1 (1%) and dose 2 (2%) are significantly different from the positive
control (10%)(p<0,05).However, the eosinophyl percentage on the test group
with dose 3 (10%) is insignificantly different from the positive control
(p>0,05). The toxicity test of the two herbs showed There are no toxicity
until dose 16 000 mg/KgBW. The conclusion are
n-hexane fraction of Phyllanthus niruri L. Herba and Taraxacum
officinale Weber et Wiggers Herba have an antiinflammation effect on mice with
Allergic Dermatitis and are safe for
oral consumption.
Keywords: Phyllanthus niruri L. Herba, Taraxacum
officinale Weber et Wiggers Herba, Mice, Antiinflammtion effect, safety
Penulis: Diana Krisanti
Jasaputra, Rosnaeni
Kode Jurnal: jpkedokterandd0700153
