PURIFIKASI DAN KARAKTERISASILINAMARASE GADUNG (Dioscorea hispida Dennst) UNTUK DETOKSIFIKASI BUBUR UMBI GADUNG
ABSTRACT: The aim of this
research was to find out purification of enzyme linamarase fromDioscorea
hispida Dennts. Enzyme purification was carried out by amonium
sulphatefractionation and Sephadex G-75 gel filtration coloumn chromatography.
Protein purifyfactor achieved was 10.5 times, protein yield was 33.3 % of the
crude cell extract proteinand specific activity was 2.93 U/mg. The enzyme
purity of each purification stage wasmonitored by SDS-PAGE and resulted one
protein band. The optimum temperature andpH of enzyme reaction was 57°C and pH
6.5 with 90 minutes incubation. Using crudelinamarin as substrat, linamarase
has Km 0.1564 μmol ml and Vmax 0.2796μmol/minute. Pure linamarase was added in
Dioscorea slurry to increase cyaniderelease.
Keywords: purification, characterization, linamarase, detoxification, Dioscorea hispidaDennts
Penulis: Harijono, Siwi N, Aji
Sutrisno
Kode Jurnal: jppertaniandd110014
