POLIMORFISME GEN GROWTH HORMONE (GH) PADA SAPI LIMURA
ABSTRACT: The research was
conducted to identify Growth Hormone (GH) olymorphism of Limura cattle. The
research was conducted in the smallholder at Lancar, Montok and Duko village,
Larangan subdistrict in Pamekasan district, East Java Province and Animal
Breeding Laboratory of Faculty of Animal Science, Gadjah Mada University in
April 2012. Then research used 35 Limura calves and 10 Madura calves (as
control). Blood samples for DNA molecular analysis, i.e. DNA isolation with
SDS-PK modification method, DNA amplification with PCR method and genotyping
with RFLP method. Identification of GH gene polymorphism was conducted by
digesting the DNA fragment of 211 bp extended from the fourth intron region (49
bp) to fifth of exon (162 bp) by AluI enzyme. The result indicated that GH gene
of Madura cattle was not polymorphic with frequencies of L allele 1.00 and V
allele 0.00. The LL genotype of Madura cattle was 1.00. Frequencies of L and V
allele in Limura cattle were 0.91 and 0.09, respectively. Limura cattle
indicated polymorphic with genotype LL 0.83 and LV 0.17. As a results, GH gene
polymorphism was found in Limura cattle with L allele frequencies higher than V
allele. Limura cattles population were not deviated from Hardy-Weinberg
quilibrium genetic condition.
Key words: Growth Hormone
Gene, Limura Cattle, Polymorphism
Penulis: Slamet Diah Vulkandari,
Tety Hartatik, Sumadi
Kode Jurnal: jppeternakandd130041
