Motilitas Spermatozoa Ayam Kampung dalam Pengencer Air Kelapa, NaCl Fisiologis dan Air Kelapa-NaCl Fisiologis pada 25-29 Derajat Celcius
ABSTRACT: This study aimed to
determine the effect of coconut water diluent, physiological NaCl and
combination of coconut water-physiological NaCl to the survival of chicken
spermatozoa at room temperature (25-29°C). Semen was collected from a local
chicken of 1.5 years old with weight of 2 kg in massage twice a week. Once
evaluated, a good quality semen was divided into 3 treatment groups: coconut
water (P1), physiological saline (P2), and its combination (P3) with the
addition of 20% egg yolk [(P1) 80:0:20 (P2) 0:80:20 and (P3) 40:40:20] and
stored at room temperature. Evaluation of spermatozoa motility conducted every
1 hour until reaching 40% of spermatozoa motility. Data obtained were analyzed
using analysis of variance (ANOVA) with design patterns Split-plot and if there
are differences between treatments tested by Duncan's multiple test. The
results showed that motility of spermatozoa is significantly different (p
<0.05) between storage time and diluent and there are interactions between
storage time and diluent. P2 is able to maintain the minimum percentage of
inseminated sperm motility (40%) for 4 hours, followed by P3 for 1 hour, and
the lowest percentage motility of spermatozoa present in P1 for 0 hours. It can
be concluded that the percentage of spermatozoa motility in group P2 is higher
than P1 and P3.
Keywords: sperm motility;
physiological saline; coconut water; local chicken
Penulis: Triva Murtina Lubis
Kode Jurnal: jppeternakandd110048
