Hubungan Polimorfisme Gen Laktoferin dengan Kualitas Susu pada Sapi Perah Friesian-Holstein
ABSTRACT: The purposes of this
study were to identify the polymorphism of the lactoferrin gene and the
association of genetic variants of the lactoferrin gene on milk quality in
Holstein-Friesian (HF) cows. The study was conducted on 194 heads of HF
lactating cows coming from semi intensive of small dairy farmers in two
villages of the KPSBU Lembang, Lembang district. Investigation on variant
genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the
lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA
(65%) and AB (35%) genotypes. Both the AA and AB genotypes had inconsistency in
controlling component of the milk quality. Further, the AB lactoferrin cows had
a higher frequency in the classification of low somatic cells count (0.75),
while the AA cows were at a higher frequency in the classification of high
somatic cells count (0.60). It was concluded that there is no strong
relationship between intron 6 lactoferrin gene with milk quality parameters.
Further, the AB genotype of the lactoferrin gene had a consistency in producing
lower somatic cells count compared to the AA genotype.
Keywords: polymorphism;
lactoferrin gene; milk quality; PCR-RFLP
Penulis: Elmy Mariana
Kode Jurnal: jppeternakandd110049
