HIDROLISIS SECARA SINERGIS PATI GARUT (Marantha arundinaceae L.) OLEH ENZIM -AMILASE, GLUKOAMILASE, DAN PULLULANASE UNTUK PRODUKSI SIRUP GLUKOSA
ABSTRACT: The purified -amylase, glucoamylase and pullulanase were used for hydrolysis of
arrowroot starch (Marantha arundinaceae L.) a local tuber from Indonesia. This
arrowroot starch is a potential source for glucose syrup production. The
amylolytic activities of α-amylase, glucoamylase (from Aspergillus
niger) and pullulanase (from Bacillus licheneformis) were 90 KNU/g, 260 GAU/g
and 390 ASPU/g, respectively. These enzymes were used to study their
synergistic effects on the hydrolysis of arrowroot starch to glucose syrup. We
have studied the effect of α-amylase concentration (0.025%, 0.045%, and 0.065%
(w/w)) and incubation time (1 hour, 1,5 hours, and 2 hours) during liquefaction
process that was combined with 24 hours of saccharification process using 0.08%
(w/w) dextrozyme (mixture of glucoamylase and pullulanase) in the glucose syrup production. This experiment showed that 1.5 hours of
liquefaction process using 0.045% (w/w) of -amylase
produced 24.64% of reducing sugar and 91.80 of dextrose equivalent. The second experiment was focused on
the determination of saccharification process, where 1 hour liquefaction
process using 0.045% (w/w) of -amylase was
combined with saccharification process using dextrozyme. In the
saccharification process, a various dextrozyme
concentrations (0.04, 0.06, and 0.08 % (w/w)) and incubation times (24 hours,
48 hours, 72 hours) were studied in relation to glucose syrup production. It was showed that 0.08% (w/w) of dextrozyme
and 24 hours of incubation time gave the best result on glucose syrup with
24.88% of reducing sugar and 92.14 of dextrose equivalent.
Keywords: arrowroot, -amylase, dextrozyme, liquefaction, saccharification, glucose syrup
Penulis: Yunianta, Tri
Sulistyo, Apriliastuti, Teti Estiasih, Siti Narsito Wulan
Kode Jurnal: jppertaniandd100014
