ABSTRACT: The determination of
pseudoephedrine hydrochloride and triprolidine hydro-chloride in influenza
syrup medicine has been performed using TLC densitometric method.
Pseudoephedrine hydrochloride and triprolidine hydrochloride were extracted using
chloroform at pH 12 from the syrup, and separated using HPTLC silica
Kie-selguhr glass plates 60 F 254, 20x10 cm2 as stationary phase, and a mixture
of methanol, ammonia and chloroform (40:2:30) as mobile phase. The plates were
analyzed using Camag TLC Scanner 3 with UV-detector at 257 nm for
pseudoephe-drine hydrochloride and at 290 nm for triprolidine hydrochloride.
The results showed that the linearity, limit of detection, and limit of
quantitation of the method for pseudoephedrine hydrochloride were 0.9999,
0.0064 µg, and 0.2124 µg respectively; while for triprolidine hydrochloride
were 0.9999, 0.0076 µg, and 0.0254 µg respectively. The coefficient of variance
(CV) of repeatability for the two substances were less than 2.0%; and the
recovery values for pseudoepherine hydrochloride and triprolidine hydrochloride
were 99.98 + 1.05% and 99.73 + 1,54% respectively. The result showed that the
samples analysed contained pseudoephedrine hydrochloride 94.36% of the labeled
ammount, and triprolidine hydrochloride 94.44% of the la-beled ammount.
Key words: pseudoephedrine
hydrochloride, triprolidine hydrochloride, TLC densitometric method.
Penulis: Hayun, Nelly D.
Leswara dan Camelia D.P. Masrijal
Kode Jurnal: jpfarmasidd070003
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