VIABILITAS DAN KERIAP Bacillus sp. BK17 DAN Enterobacter sp. BK15 PADA SUMBER KARBON DAN NITROGEN YANG BERBEDA
Abstract: To propagate bacterial cell for biocontrol purpose, suitable
nutrient have to be determined in which carbon and nitrogen source was often as
limited factor of bacterial growth. Proper storage for biocontrol agent such as
bacterial cell should also be considered in order to keep the cell viable when
used. The aim of this study is to find out suitable carbon and nitrogen sources
for viability and swarming of
chitinolitic bacterial Bacillus sp.
BK17 and Enterobacter
sp. BK15. The
highest population of
bacterial growth (3.7x108 cfu/ml)
was found in molases-sodium nitrate
(MS) medium and
the lowest population
was found in
crab shell-sodium nitrate (CS)
growth (2.4x108 cfu/ml) after 25 days of incubation. The swarming
activity of the isolates were varied
to some extent
with the highest
was 51 mm
in 2% agar
molases-urea after 5 days of
incubation. Molases-sodium nitrate (MS) medium is suitable carbon and
nitrogen source for the viability of Bacillus sp. and Enterobacter sp.
Meanwhile, agar molases-urea medium with 2% agar is suitable medium for
swarming ability for both bacteria.
Keywords: Bacillus, C and N-source, Enterobacter, swarming, viability
Penulis: Sirma Novita Nasrah, Dwi Suryanto, It Jamilah
Kode Jurnal: jpbiologidd120027
