Abstract: A research has been
conducted in testing the enzymatic activity of the encapsulated Lactate dehydrogenase
(LDH) in silica by sol-gel process using sodium silicate from rice hull ash.
The encapsulation was carried out by mixing sodium silicate (Na2SiO3) sol
solution and phosphate buffer pH 7 containing both LDH enzyme and coenzyme of
nicotinamide adenine dinucleotide (NAD+). After 2-6 minute of shaking, the
mixture was then transferred into a 96-microwell plate and stored overnight.
The encapsulation activity of encapsulation product was quantified by measuring
absorption of NADH, as coenzyme hydrogenation product, at 340 nm utilizing an ELISA
(Enzyme Linked Immunosorbent Assay) reader. For comparison similar experiment
was also carried out for free enzyme. The result of the research shows that the
kkat for encapsulated LDH enzyme (ET) is smaller than free enzyme (EB), which
means that the encapsulation enzyme will affect the velocity of product
reaction. The velocity of the encapsulation enzyme (ET) reaction is lower than
free enzyme (EB) as the consequence of weak interaction between the substrate
and enzyme or influenced by k1.
Keywords: enzyme
encapsulation, lactate dehydrogenase, rice hull ash, sol-gel process
Penulis: Noor Hindryawati
Kode Jurnal: jpkimiadd100055
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