Abstract: One of the alternative utilization of pineapple peel waste is
to convert them to lactate acid via fermentation. This research is aimed to
obtain better understanding on the growth of lactate bacteria (Lactobacillus
plantarum) by varying substrate
concentration: 100 g/L - 600
g/L and stirring
rate of 50
rpm, 150 rpm, and 250
rpm. The growth
of anaeroic process was performed
without pH controlling with initial pH of 5 and temperature of 28OC. Pineapple peel was used as the substrate
after romoving water content using ammonium dihydrogen phosphate. Cell growth was analyzed by calculating the
total of microorganism cell using hemocytometer. In
order to determine
lactate acid production,
glucose concentration was analyzed
using spectrophotometer. Nelson-Somogy
method was employed in
this analysis. Ethanol
concentration was also
analyzed by calculating
the titration of acid lactate concentration. The optimum growth of
Lactobacillus plantarum was found at 28oC, stirring rate of 150 rpm and
substrate concentration of 500 g/L contributing to the total cell of
3,85.108, 1,13.109, and
4,00.108 for lag, exponential,
and decrease phase respectively. Further
analysis carried out
based on stirring
rate of 150
rpm and substrate concentration
of 500 g/L resulted in the largest average concentration of lactate acid of
1.620% v/v with glucose consumption percentage of 51,76%.
Keywords: pineapple peel, Lactobacillus plantarum, lactate acid
Penulis: Panca Nugrahini Febriningrum
Kode Jurnal: jpkimiadd130109
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