GAMBARAN HISTOLOGIS PULMO MENCIT JANTAN (Mus musculus L.) SETELAH DIPAPARI ASAP ROKOK ELEKTRIK
Abstract: The effects
of electric cigarette
exposure on lung
tissue of mice
were studied. The
experiments using completely
randomized design (CRD) with 3 treatments, i.e.
treatment of electric cigarette with strawberry flavour, tobacco
flavour, and control
treatment that was
not exposed to
cigarette smoke. Eight
male mice were used for each
group. Exposure treatment was done every day for 2 consecutive weeks with dosage of 20 times
suction using syringe
of 60 mL.
After receiving treatment
for 2 weeks,
the mice were
killed by cervical dislocation.
Lung organ was
isolated and prepared
histologically using paraffin
method and then stained
with haematoxylin and
eosin (HE). Result
showed, treatment mice
lose weight significantly
(p< 0.05). In gross anatomy, electric cigarrete smoke affect lung
feature, but histological observation showed that membrane damage,
damage to the lumen
and the relationship
between alveoli were
statistically unsignificant
(p>0.05). In summary,
short period exposure
to electric cigarette smoke
tends to induce
injury to lung
at anatomical level,
but in several
histological aspects electric cigarette treatment has
no significant effect on lung
tissue.
Keywords: alveolar lumen,
alveolar membrane, electric cigarettes
Penulis: Nanin Triana,
Syafruddin Ilyas dan Salomo Hutahaean
Kode Jurnal: jpbiologidd130006
