Induction of Rat Germ Cell Apoptosis by Testosterone Undecanoate and Depot Medroxyprogesterone Acetate and Correlation of Apoptotic Cells with Sperm Concentration
ABSTRACT: to investigate the
safety and effectiveness of dose TU+DMPA hormones in developing potential male contraceptives.
Methods: suppression of rat sperm concentration through increased apoptotic
germ cells by in vivo administration of a long-acting androgen composed of a
combination of testosterone undecanoate (TU) and depot medroxyprogesterone
acetate (DMPA) was performed. Adult Sprague-Dawley rats received 2.5 mg TU
every 6 week and 1.25 mg DMPA every 12 week for 60 week, a regimen known to
rapidly reduce testosterone production by the testes and produce azoospermia
within 12 week. Sperm concentration data were log transformed before analysis.
Results are expressed as the mean±SEM. ANOVA, followed by post hoc test was used
to determine differences across time and phase. T test was employed to
determine differences between two groups. Results: apoptosis revealed
signifcant increase in apoptotic germ cells (80% when rats were administered with
TU+DMPA. Apoptotic germ cells can be found in several spermatogonia (20%),
spermatocytes (30%), and spermatids (50%). Conclusion: dose TU+DMPA hormones
may be a safe and effective way to develop potential male contraceptives.
Author: Syafruddin Ilyas, Silvia
W. Lestari, Nukman Moeloek, Asmarinah, Nurjati C. Siregar
Journal Code: jpkedokterangg130251
