Association Between the Low Levels of Vitamin D and Treg Function in Systemic Lupus Erythematosus Patients
ABSTRACT: to determine the
relationships between vitamin D and CCR4 expression on Treg, and Treg migratory
capacity in SLE patients in Indonesia. Methods: vitamin D level assesment, CCR4
expression on Treg and Treg migratory capacity were performed on 41 SLE
patients and 20 healthy controls. Serum vitamin D levels were measured by ELISA
(Cusabio). The expression of CCR4 on Treg was detected by flow cytometry and Treg
migratory capacity was performed in chemotaxis chamber using CCR4 ligands, TARC
and MDC, and subsequently analyzed by flow cytometry using FACS Calibur (Becton
Dickinson). The number of migrated Treg is the ratio of CD4+CD25+CCR4+ cells
number in lower chamber of chemotaxis compared to the total number of
CD4+CD25+CCR4+ cells before migration, and stated in percentage. Results:
vitamin D levels were signifcantly lower in SLE patients compared with healthy
controls (p=0.000). The vitamin D levels were positively correlated to the
percentage of migrated Treg toward TARC (p=0.015) and MDC (p=0.000), but there
was no difference in Treg CCR4 expression between SLE patients and healthy
controls. Conclusion: low vitamin D levels cause reduced Treg migratory
capacity in SLE patients and healthy people. This influence occurs through other
factors rather than CCR4 expression.
Author: Kusworini Handono,
Dona Marisa, Handono Kalim
Journal Code: jpkedokterangg130250
