Distribution of Dimethylarginine-DimethylaminohydrolaseII (DDAH2) Gene Polymorphism in Hemodialysis Patients

ABSTRACT: to describe the distribution of single nucleotide polymorphisms (SNPs) at -449 (promoter region) DDAH 2 in hemodialysis (HD) patients. Methods: this study was a descriptive study, 56 HD patients and 30 healthy individuals were enrolled. Based on its etiology, the HD patient group was further divided into hypertension (HT) group and non-HT group. DNA was extracted from whole blood samples with a commercially available DNA isolation kit. Genotyping of the polymorphisms was performed using PCR-based SNP detection methods (Applied Biosystems, Carlsbad, USA) based on 5’-exonuclease activity assays for rs805305 (-449 G/C). Allelic variation was assessed in each participant. Results: heterozygotes were observed as the most abundant genotypes in both groups (70% in healthy individuals and 55% in HD patients), followed by GG genotype in the HD patients (30%), while CC (27%) was the second most common genotype polymorphism in the healthy individuals. Conclusion: there is a signifcant difference in distribution of DDAH 2 gene polymorphism among HD patients compared to healthy individuals (p=0.01).
Key words: CKD, DDAH2 gene polymorphism, hemodialysis, hypertension
Author: Muhammad Taha, Muhammad Yusuf, Maulana A. Empitu, Achmad Bakarman, Yasuhiko Tomino
Journal Code: jpkedokterangg130254

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