Distribution of Dimethylarginine-DimethylaminohydrolaseII (DDAH2) Gene Polymorphism in Hemodialysis Patients
ABSTRACT: to describe the
distribution of single nucleotide polymorphisms (SNPs) at -449 (promoter
region) DDAH 2 in hemodialysis (HD) patients. Methods: this study was a
descriptive study, 56 HD patients and 30 healthy individuals were enrolled.
Based on its etiology, the HD patient group was further divided into
hypertension (HT) group and non-HT group. DNA was extracted from whole blood
samples with a commercially available DNA isolation kit. Genotyping of the
polymorphisms was performed using PCR-based SNP detection methods (Applied
Biosystems, Carlsbad, USA) based on 5’-exonuclease activity assays for rs805305
(-449 G/C). Allelic variation was assessed in each participant. Results:
heterozygotes were observed as the most abundant genotypes in both groups (70%
in healthy individuals and 55% in HD patients), followed by GG genotype in the
HD patients (30%), while CC (27%) was the second most common genotype
polymorphism in the healthy individuals. Conclusion: there is a signifcant
difference in distribution of DDAH 2 gene polymorphism among HD patients
compared to healthy individuals (p=0.01).
Key words: CKD, DDAH2 gene
polymorphism, hemodialysis, hypertension
Journal Code: jpkedokterangg130254
