The Role of Anti-dengue Virus NS-1 and Anti-protein Disulfde Isomerase Antibodies on Platelet Aggregation in Secondary Dengue Infection
ABSTRACT: to observe the
correlation between anti-NS-1 and anti PDI antibodies against platelets
function disorder on secondary dengue infection. Methods: 50 patients with
secondary DV infection according to WHO criteria were observed by a cross
sectional study. Patient’s blood was collected on day 3, 5 and 7 after fever onset.
Platelets aggregation test was done to prove the possibility of platelets dysfunction.
Anti-NS-1 and antiPDI antibodies were determined by solid phase ELISA. Results:
the inhibition of platelets aggregation was increased among day of observation.
Means value of inhibition on day 3 is 46.6%, day 5 is 52.5% and day 7 is 56%.
There is a signifcant difference (p<0.05) of inhibition of platelet
aggregation value between days of observation. The antibodies against NS-1 DV
and PDI were detected in all 50 sera with the positive rate of 90% develop NS-1
antibodies and 72% of PDI antibodies, on day 3 of symptoms. The highest OD of
NS-1 antibodies is detected on the day 3 and decreased on day 7. The OD of PDI
antibodies was increased on day 3 and still increasing on day 7. There is a
signifcant correlation between anti NS-1 and PDI antibodies (r=0.386-0.490), while
the differe nces of OD between observation days are not signifcant (p>0.05).
Conclusion: the kineticsprofle of NS1 and PDI antibodies responses, which were
detected by the third day of symptoms. Dengue patients’ sera inhibited
platelets aggregation. NS-1 antibodies and PDI antibodies might have a role on
the platelets aggregation dysfunction; however, there is no correlation between
them. It is possible that other mechanism involve in the inhibition of
platelets aggregation.
Key words: anti-NS-1
antibodies, anti PDI antibodies, inhibition platelets aggregation, secondary
dengue infection
Author: Andhika Rachman, A.R.
Harahap, R.M. Widhyasih
Journal Code: jpkedokterangg130253
