VALIDATION OF UV-VIS SPECTROPHOTOMETRIC METHOD FOR SOLUBILITY STUDY OF PENTAGAMAVUNON-0 IN SELF-NANOEMULSIFING DRUG DELIVERY SYSTEM VEHICLES
Abstract: Pentagamavunon-0 has
a potent anti-inflammatory activity but has poor solubility and absorption
properties, which can be improved by SNEDDS formulation. One of the most
critical parameters of the optimization of SNEDDS is the solubility of PGV-0 in
various oils, surfactants, co-surfactant, and in the final product. This study
is aimed to validate a simple and inexpensive UV-Vis spectrophotometric method
for PGV-0 quantification in SNEDDS formulation. The assay was performed using
UV-Vis spectrophotometer, methanol as a solvent, and Vis detection of 419 nm.
The linearity, limit of detection (LOD), limit of quantity (LOQ), precision,
and accuracy of the method was determined. The solubility study was performed
by modified shake flask method, using labrasol as a vehicle model. The
insoluble PGV-0 was separated and cleaned from the filtrate, quantified by
validated UV-Vis spectrophotometry, and the solubility of PGV-0 was determined.
Validation measurement was carried out in the range 1-8 ppm. The results showed
a good linearity with r=0,99949, LOD=0.29 ppm, and LOQ=0.95 ppm. The accuracy
expressed as recoveries were found to be 98.32 - 100.74%. The precision was
good (RSD=1.29 – 1.93%). The recovery of solubility of PGV-0 was 100.02%
indicated a good accuracy. This method can be used for the solubility test of
many samples as in the SNEDDS formulation.
Keywords: validation; UV-Vis
spectrophotometry; PGV-0; solubility study
Penulis: Ika Yuni Astuti
Kode Jurnal: jpfarmasidd170471
