Uji Daya Hambat Ekstrak Etanol Daun Puding Hitam (Graptophylum pictum L. Giff) pada Pertumbuhan Bakteri Pseudomonas aeruginosa


ABSTRACT: One of the bacteria that cause infections is Pseudomonas aeruginosa. The most common infection treatment is administration of antibacterial. Pseudomonas aeruginosa resistant to many antibacterial that will multiple if not in the selection of antibacterial. One of the plants is a tradisional treatment as antibacterial is Puding Hitam Leaves (Graptophyllum pictum (L) Griff). This study aims to determine the inhibitory ethanol of extract Puding Hitam Leaves (Graptophyllum pictum (L) Griff) on the growth of Pseudomonas aeruginosa. Inhibition test performed by disc diffusion method with four variations dose that is 120 ug / mL, 230 ug / mL, 470ug / mL and 940ug / mL. Chloramphenicol 0.3% as positive controls and negative control is DMSO 10%. The results showed extract of Puding Hitam Leaves is able to inhibit the growth of bacteria Pseudomonas aeruginosa with a diameter inhibitory consecutively dose 120 ug/mL, 230 ug/mL, 470ug/mL and 940ug/mL that is 11.5 mm, 14.6 mm, 18.7 mm, 15.3 mm, 23.7 mm. One­Way ANOVA statistical tests, showed each treatment has a significant difference (p <0.05) except between extract 230 ug / mL and 940 ug / mL (p> 0.05). One­Way ANOVA statistical tests, showed each treatment has a significant difference (p <0.05) except between extract 230 ug / mL and 940 ug / mL (p> 0.05).
Keyword: Extract, Pseudomonas aeruginosa, Inhibitory
Penulis: Setya Enti Rikomah, Yuska Novi yanti, Wahyu Juarsah
Kode Jurnal: jpfarmasidd170093

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