The Role of Fennel Infusion on Estrous Cycle and Follicles Development of White Rats
Abstract: This study aims to
describe the estrous cycle and follicles development in female rats given
infusion of fennel fruit that known to have phytoestrogen compound. Twenty five
of female rats were used in this research and were divided into 5 groups. Group
I was negative control group (NC), given 1 mL/100g bw of distilled water, group
II was positive control group (PC), given 0.0045 mg/100g bw of ethinyl
estradiol, groups III, IV and V were treatment groups (TI, T2 and T3), given
fennel infusion at 36.5; 73 and 146 mg/100g bw respectively. All treatments
were conducted every morning for 20 days by oral route. Changes of vaginal
epithelium were observed through vaginal swabs previously stained with Giemsa
stain. Histopathological examination of ovarian swere examined to reveal
follicles development. Results showed that fennel fruit infusion extended the
duration of estrous and metestrous phases, while shortened the proestrous and
diestrous phases. Eventhough the longest estrous phase was found in T3 group,
there was no significant effect on the lengthening of estrous cycle. Moreover,
infusion of fennel fruit had no effect on the development of ovarian follicles,
except tended to increase the number of corpus luteum.
Keywords: phytoestrogen,
fennel fruit, estrous cycle, follicles development, rat
Penulis: HERA MAHESHWARI
Kode Jurnal: jpfarmasidd160433
