The Effectiveness of GABA Agonist in Decreasing Expression of NR2B Subunit of N-Methyl-D-Aspartate (NMDA) Receptor in Neuropathic Mice by Partial Sciatic Nerve Ligation (PSNL) Method
Abstract: Neuropathy pain is a
pain that caused by nerves injury. Nowadays, treatment for neuropathic pain
change to drugs that works as GABA agonist and cause reimbalance between excitatory
and inhibitory neurotransmitter in central nervous system (CNS). The present
study was designed to investigate the effectiveness of gabapentin and baclofen
in decreasing N-Methyl-D-Aspartate (NMDA) receptor NR2B subunit activity in
neuropathic pain. Fourty mice were divided into 8 groups i.e sham, negative
control, gabapentin (10, 30, 100 nmol) and baclofen (1, 10, 30 nmol).
Neuropathic pain was induced by ligation of sciatic nerve with Partial Sciatic
Nerve Ligation (PSNL) method). Treatments were administrated intrathecally once
a day for seven consecutive days, at a week after induction. On day 15th, mice
were sacrified and the spinal cord were removed quickly. The expression of NMDA
receptor NR2B subunit were examined with imunohistochemistry and data were
analyzed by one way anova. The result from this research was gabapentin and
baclofen administration significantly decrease expression of NMDA receptor NR2B
subunit in mice compared to sham group. The higher the dose, the more effective
to decrease the number of neuron that express NR2B. The conclusion of this
research was gabapentin and baclofen treat neuropatic pain by decreased the
number of NMDA receptor NR2B subunit.
Keywords: Neuropathic pain,
PSNL, baclofen, gabapentin, spinal cord
Penulis: FIFTEEN APRILA FAJRIN
Kode Jurnal: jpfarmasidd160426
