SECONDARY BIOACTIVE METABOLITE GENE CLUSTERS IDENTIFICATION OF ANTICANDIDA-PRODUCING Streptomyces Sp. GMR22 ISOLATED FROM WANAGAMA FOREST AS REVEALED BY GENOME MINING APPROACH
Abstract: Streptomyces are a
group of Gram-positive bacteria belonging to the Actinobacteria class, which
are among the most important bacteria for producing secondary bioactive
metabolites such as antibiotics, chemotherapeutics, insecticides and other
high-value chemicals. Genome mining of gene clusters that encode the
biosynthetic pathways for these metabolites has become a key methodology for
novel compound discovery. Recently, we have isolated the Streptomyces sp. GMR22
from Cajuput rhizospheric soil at Wanagama Forest, Indonesia. GMR22 produced
secondary metabolite that inhibited Candida albicans with IC50 of 62,5 μg/mL.
The objective of this work was to reveal the novel secondary metabolites from
GMR22 by genome mining approach. The antiSMASH 3.0 was used to predict gene
clusters that encode the biosynthetic pathways of secondary metabolites in the
genome of GMR22, and their core chemical structures. The pylogenomic analysis
showed that GMR22 was closely related to Streptomyces bingchenggensis BCW1, as
well as to the large genome size (9.5-12.7Mbp) groups of Streptomyces.
AntiSMASH 3.0 analysis revealed that the genome of Streptomyces sp. GMR22
harbored at least 63 gene clusters that encode the biosynthetic pathways of
secondary metabolites. It was the highest number of gene clusters had been
observed among the members of Streptomyces groups, with PKS was predicted as
the major groups of the identified gene cluster products. The results suggested
that GMR22 could be a strong potential candidate of secondary bioactive
metabolites source.
Keywords: Streptomyces sp.
GMR22; antifungal; genome sequence; antiSMASH
Author: Camelia Herdini, Sofia
Mubarika, Bambang Hariwiyanto, Nastiti Wijayanti, Akira Hosoyama, Atsushi Yamazoe,
Hideaki Nojiri, Jaka Widada
Jounral Code: jpfarmasigg170009
