PROFIL BIOAUTOGRAM AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT JAMUR LAUT PADA ALGAE Kappaphycus alvarezii SECARA KLT BIOAUTOGRAFI
ABSTRACT: The Research
Chromatogram Profile Of Activities Antibacterial Extracts Isolates Marine Fungi
in Algae Kappaphycus alvarezii by TLC Bioautography has been done with the
intention of doing the screening test of pathogenic bacteria that can be
inhibited by the extract of F3 (extract liquid culture and extract mycelia) and
extracts of E (extract liquid culture and extract mycelia ) and see
chromatogram profile of liquid culture extract of F3 on bacteria Escherichia
coli and mycelia extract of E on bacteria Bacillus subtilis. This research was
conducted purification of Fungi isolates E and F3 followed by fermentation
process of fungi isolates for 3 weeks. Then proceed with the extraction process
using an ethyl acetate solvent. The solvent was evaporated to obtain a dry
extract, followed by a screening test of antibacterial activity against
bacterial pathogenic, antibacterial activity test by TLC Bioautography to
liquid culture extract of F3 and extract mycelia of E. The results of the
screening test with a concentration of 250 µg was obtained to extract liquid
culture of F3 and E provide activity against 5 pathogenic bacteria to extract
mycelia while F3 and E provide activity against two bacterial pathogens. Separation
of compounds by TLC using the ethyl acetate eluent: ethanol: water (8: 2: 1).
The result of TLC Bioautography test of liquid culture extract of F3 to
bacteria Escherichia coli and mycelia extract of E on bacteria Bacillus
subtilis showed the existence of antibacterial activity.
Key words: Alga, Antibakteri,
TLC-Bioautografi, Isolate Fungi
Penulis: Fitriana, Rusli
Kode Jurnal: jpfarmasidd170419
