Positive Impact of Ethyl Acetate Fraction of Kalanchoe pinnata on Anti-Smith Antibody and T Reg in Lupus Mice
Abstract: Kalanchoe pinnata
(Lmk) Pers is a medicinal plant that has many activities, such as antioxidant, anti-infl
ammation and immunosuppressant activities. Based on our previous study, aqueous
extract ofthis plant leaves has a repairing eff ect on lupus nephritis model.
The expected active compounds are fl avonoids. Those compounds are separated in
the ethyl acetate fraction. Therefore, this study observed the activity of the
ethyl acetate fraction of Kalanchoe pinnata (Lmk) Pers on reducing the level of
AntiSmith autoantibody and the regulation function of CD4+CD25+ T reg in lupus
mice. The experimental groups were a negative control group that received
placebo, ethyl acetate fraction of Kalanchoe pinnata (Lmk) Pers (EF-KP) group
and a positive control group that received cyclophosphamide. The anti-Sm level
was measured by using indirect ELISA. The spleen cells were prepared for
CD4+CD25+ T reg assay using fl ow cytometry. The result of this experiment was
a reduction of anti-Sm antibody level. The decrease was suffi cient to
stabilize lupus condition. The fl ow cytometry assay results showed the increase
in the relative percentage of CD4+CD25+ T reg. This function can inhibit the
reactivity of autoantigens, so it prevents tissue and organ damage from lupus.
However, further research is needed to observe the complete mechanism.
Keywords: Kalanchoe pinnata
(Lmk) Pers, lupus, anti-Sm, CD4+CD25+ T reg, immunosuppressant
Penulis: NIKEN INDRIYANTI,
JUNAIDI KHOTIB, JOEWONO SOEROSO
Kode Jurnal: jpfarmasidd170184
