PENETAPAN KADAR TANIN TOTAL EKSTRAK BIJI JINTAN HITAM (Nigella sativa) SECARA SPEKTROFOTOMETRI UV-VIS
Abstrak: Researched about The
Total Tannin Assay Extract of Black Cumin Seeds (Nigella sativa) with
Ultraviolet-Visible Spectrophotometry. This research aims to determine the
levels of total tannin extract of black cumin seeds (Nigella sativa). The
method used is multilevel macerationusing n-hexane and ethanol 70%. Extracts
were identified using FeCl3reagent and gelatin solution showed positive
resultscontaining tannin onthe ethanol70%extract.The extracts thatpositive
containing tannins diluted, added Folin Deanis reagent and saturated sodium
carbonate, then read at 740 nm
wavelength. Forming reaction was the oxidation reduction where tannin as
reductant and Folin Denis as an oxidant. The results of oxidation will form a
blue color that can be read at maximum wavelength. Based on the regression y =
0.004 x + 0.097 and 0.996 for determination coefficient, was gained an average
of tannin levels in the ethanol extract of black cumin seeds (Nigella sativa)
is 4, 13 %.
KATA KUNCI: Black Cumin
Seeds., Tannin., Ultraviolet-Visible Spectrophotometry
Penulis: Mukhriani, Faridha Yenny Nonci, Mumang
Kode Jurnal: jpfarmasidd140555
