Optimum Conditions for Extraction of Antibacterial Compounds from Citrus Aurantifolia Fruit Peel Waste

Abstract: Citrus fruit peel is a major waste in citrus fruit processing industry. The research on extraction active compounds of Citrus aurantifolia (lime) fruit peel waste and antibacterial activity assay has been done. The aim of research was to get optimum condition to extract their active compounds which have antibacterial activity. The dried lime fruit peel was extracted by maceration method using ethanol 48%, 72%, and 96%. The dried and fresh lime fruit peel were also extracted using ethyl acetate. Antibacterial assay was done by diffusion agar against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The result showed that optimal condition to extract antibacterial compound using fresh sample with ethyl acetate as solvent. The ethyl acetate extract of fresh sample was more active against S. aureus than E. coli.
Keywords: extraction, antibacterial, Citrus aurantifolia, fruit peel
Penulis: Rima Munawaroh
Kode Jurnal: jpfarmasidd170543

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